The effect of pentoxifylline on renal ischemia and reperfusion damage
Orhan DEMİRTAŞ1, Mete KAYA1, M. Emin BOLEKEN<1, Turan KANMAZ1, Hakim ÇELİK 1, Füsun BABA 2, Özcan EREL 1, Selçuk YÜCESAN1
1Harran Üniversitesi Tıp Fakültesi Çocuk Cerrahisi, Biyokimya Anabilim Dalları, Şanlıurfa
2Harran Üniversitesi Tıp Fakültesi Çocuk Cerrahisi, Patoloji Anabilim Dalları, Şanlıurfa
Keywords: Renal ischemia and reperfusion, oxidative stress, pentoxifylline
Abstract
Aim: To investigate the effect of pentoxifylline (Ptx) on oxidative and antioxidative status in the renal ischemia and reperfusion (I/R).
Method: Twenty four Wistar albino rats were used for the study. Rats were divided into four categories as control, ischemia, I/R and I/R plus Ptx administration groups. Blood samples and renal tissue specimes were obtained from rats after the operations. As oxidants malondialdehyde (MDA) and total peroxide levels, and total antioxidants capacity (TAOC) were measured from the samples. The results were analyzed statistically. In addition, renal tissue sepcimens were examined histopathologically.
Results: Decreased blood and renal tissue MDA and t-prx levels were found to be significantly lower in ptx group when compared with other groups (p<0.05). Blood TAOC levels in Ptx group were significantly lower than control and I/R groups. No significant difference was found in tissue TAOC levels between groups. Histopathologically, in the I/R group. kidneys were found to be damaged.
Conclusions: The oxidative stress and renal damage after renal I/R were found to be more severe relative to ischemic harm. However this damage could be reduced by Ptx administration.