Mesenchymal stem cell therapy in corrosive esophagitis in rats
Hatice Sonay Yalçın Cömert1, İbrahim Ötgün2, Abdülkerim Temİz3, İlknur Kozanoğlu4, Zerrin Yılmaz5, Eylem Akar Özkan6, Akgün Hİçsönmez3
1Karadeniz Teknik Üniversitesi, Tıp Fakültesi, Çocuk Cerrahisi Anabilim Dalı, Trabzon
2Memorial Ankara Hastanesi, Çocuk Cerrahisi Anabilim Dalı, Ankara
3Başkent Üniversitesi Tıp Fakültesi, Çocuk Cerrahisi Anabilim Dalı, Ankara
4Başkent Üniversitesi Tıp Fakültesi, Fizyoloji Anabilim Dalı, Ankara
5Başkent Üniversitesi Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Ankara
6Özel Anadolu Sağlık Merkezi, Patoloji Anabilim Dalı, Gebze
Keywords: Corrosives, esophagitis, mesenchymal stem cells, fibrosis
Abstract
Objective: The aim of this study was to investigate the efficacy of procure mesenchymal stem cells (MSCs) in tissue culture taken from a volunteer donor’s bone marrow which were marked and then implanted in a model of corrosive esophagitis in rats.
Material and Methods: Cell samples from a volunteer male human donor’s bone marrow aspiration were prepared for 3 weeks in the cell culture and then they were split in two for flow cytometry and differentiation (adipogenic and osteogenic). Before injection to the rats cells were marked with 1’-Dioctadecyl3,3,3’,3’-tetramethylindocarbocyanine (DiI). Thirty WistarAlbino female rats were divided into three equal groups. A standard corrosives esophagitis model was produced by 15% sodium hydroxide (NaOH). Group I (sham group): Only used 0.9% saline and did not form any esophagitis. Group II: Applied only corrosives esophagitis model. Group III: Applied corrosives esophagitis model and two hours later marked MSCs have been implanted. We assessed the esophageal tissue by measuring histopathologic damage, stenosis index (SI), for the cells with marked DiI by fluorescence microscope and fluorescence in situ hybridization (FISH) for Y chromosome.
Results: We have indicated MSCs with flow cytometry. We have represented adipogenic and osteogenic differentiation by using differentiation techniques. The histopathologic parameters were examined by fibrosis, inflammation and necrosis. There was a significant difference between the groups in fibrosis (p<0.05). There was not significant difference between the groups for SI. DiI marked stem cells were shown with examination by fluorescence microscope. Finally the Y chromosomes were determined in the esophageal tissue of rats by FISH analysis except one rat.
Conclusion: Results demonstrated that after forming a corrosive esophagitis model in rats; MSCs can be implanted to esophageal epithelium and have partly curative effect by reducing fibrosis. There was no significant difference with other histopathologic findings. Therefore more detailed studies are needed using MSCs in corrosive esophageal injuries.