Effect of oral immunoglobulin G in experimental necrotizing enterocolitis model
Müslim YURTÇU, Bahattin AYDOĞDU, Hatice TOY, Mehmet GÜRBİLEK, Engin GÜNEL
Selçuk Üniversitesi, Meram Tıp Fakültesi, Çocuk Cerrahisi, Patoloji ve Biyokimya Anabilim Dalları, Konya
Keywords: Necrotizing enterocolitis, newborn, immunoglobulin G, treatment
Abstract
Aim: Investigation of the protective effect of oral immunglobulin(Ig) G on rat intestinum in experimental necrotizing enterocolitis model.
Materials and Methods: 40 newborn rats were devided into 4 groups each containing 10 rats. While control (C) group was fed by breast, the rats in necrotizing enterocolitis (N), sham (S), and treatment (T) groups were settled into incubators at 36ºC and 60 % humidity and fed, but not by breast. The rats in group C were breast fed. The rats in N group were fed with Formula as soon as they were born. The rats in T group were fed with Formula and 1200 mg/kg/day oral Ig A with 4-hour intervals. The rats in S group were fed with Formula and 0.1 ml/kg/day distilled water which is solvent of Ig. The rats in all groups were weighed and sacrified on fourth day. 2 cm intestinal segment from proximal of ileocaecal valve was used for histopathologic examination, another 10 cm intestinal segment for biochemical examination. After laparotomy, H&E was used for histopathological examination and apoptosis repressor with card Ab-1 citt for immunohistochemical examination. Biochemical parameters such as TNF-α, and IL-6 were evaluated.
Results: The rate of mortality in N, S, and T groups was significantly higher than groups C (P=0.038). Significant weight increase in group C was significantly higher than N, S, and T groups (P=0.000). Histopathologic parameters such as villus injury, transmural necrosis in group C were found to be significantly decreased compared with N, S, and T groups according to scoring system (P=0.000). Apoptosis in group C was found to be significantly decreased compared with N, S, and T groups according to scoring system (P=0.001). IL-6 and TNF-α levels were identified to be significantly decreased in group C compared with N, S, and T groups (P< 0.05). There was no significant difference among N, S, and T groups (P>0.05).
Conclusion: Pure IgG given orally was not identified to decrease intestinal damage and to prevent NEC in experimental NEC model both biochemically and histopathologically.