Effect of carbondioxide insufflation on bowel in an experi- mental NEC model | 2018, Volume 32 - Issue 1

Şenol EMRE¹, Gonca TEKANT¹, Dildar KONUKOĞLU², Sinem FIRTINA², Sergülen DERVİŞOĞLU³,Günay CAN⁴, Cenk BÜYÜKÜNAL¹

¹İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi Çocuk Cerrahisi Anabilim Dalı, İstanbul, Türkiye
²İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi Tıbbi Biyokimya Anabilim Dalı, İstanbul, Türkiye
³İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi Tıbbi Patoloji Anabilim Dalı, İstanbul, Türkiye
⁴İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi Halk Sağlığı Anabilim Dalı, İstanbul, Türkiye

Keywords: Necrotising enterocolitis, laparoscopy, pneumope- ritoneum

Abstract

Aim: The aim of the study is to evaluate the effects of laparoscopy, which is reported to be safely applied in newborns with NEC, on the gastrointestinal tract in a animal model.

Material and Methods: Forty newborn Sprague-Dawley rats were divided into four different groups of ten. Group K was the control group with no intervention whereas Group P was subjected to pneumoperitoneum using carbon dioxide insufflation under 10 mmHg for 30 minutes followed by desufflation for 30 minutes on the fourth day of the study. Rats in Group N were NEC models established by the method described by Okur. Group NP was subjected to pneumoperitoneum-desufflation on the fourth day of the study as Group P in addition to the procedures applied on Group N. Subjects were decapitated on the fourth day and histopathological findings of the resected terminal ileum, and nitric oxide, glutathione and TBARS levels were investigated . Statistical evaluation of biochemical results were was performed using ANOVA and Tukey HSD tests, whereas Mann Whitney-U and Kruskal -Wallis tests were used for the evaluation of histopathological results.

Results: TBARS levels were significantly higher in Group NP compared to Groups K and P. However, a significant difference was not determined between the other groups. NO levels were significantly higher in Group NP than in Group K. There was no significant difference between the other groups. When GSH levels were compared, Group NP was found to have significantly higher GSH levels than Groups K, P and N. While any significant difference was not found between Groups 1, and N. Additionally, GSH levels were significantly higher in Group N when compared with Groups K and P. Histopathological stages of Groups P, N and NP were found to be significantly higher, whereas no difference was observed among these groups.

Conclusion: In the study, we demonstrated that carbondioxide pneumoperitoneum does not have a negative contributing effect on the tissue damage seen in NEC. The results of this animal model study support the safety of diagnostic laparascopy, a newly used method, in NEC.