Abstract

Aim: An experimental study was performed to evaluate the protective effect of platelet-derivated-growth factor inhibitor (Trapidil) in ischemia reperfusion injury in intussusception.

Methods: Sixty Wistar albino rats, weighing 200-250 g in both sexes were enrolled into study. In control group (CG, n:15), 2 cm of intestinal segment, 20 cm proximal to ileoceacal valve was removed after intraperitoneal NaCl infusion. In intussusception group, intussusception (IG, n:15) was performed at 20 cm proximal to ileoceacal valve by pushing the proximal intestinal segment distally through a stile. Two cm of intestinal segment was removed 4 hours after intussusception. Intussusception-reperfusion group (IRG, n:15) was obtained after performing intussusception similar to IG and allowed to perfusion 4 hours after intussusception. In this group, intestinal segment was removed 4 hours after reperfusion. In the last group (TG, n:15), Trapidil (40 mg/kg) was administered intraperitoneally, one hour before reperfusion, after achiving an ischemia/reperfusion injury similiar to IRG. Two cm of intestinal segments, 20 cm proximal to ileoceacal valve, were removed for histopathologic evaluation. The specimens were also evaluated for oxidative injury markers (catalase, glutathione peroxidaseG-px, superoxide dismutase-SOD, and malondialdehydeMDA).

Results: Although histopathologic evaluations revealed no intestinal injury in CG, other groups showed higher grades of intestinal injury (p<0.05). Though, there was no difference in histopathologic grades between IG, IRG and TG groups. MDA levels were significantly increased in experiment groups when compared to CG. But, significant alteration in MDA levels was detected in TG, when compared to IRG (p=0.00). Antioxidant enzymes; Catalase, G-px and SOD, significantly decreased in IG and IRG. Trapidil administration increased the levels of these three enzymes when compared to IRG (p=0.005, p=0.036, p=.0.02 respectively).

Conclusion: Trapidil prevents ischemia-reperfusion injury in intussusception by increasing catalase, G-px, SOD levels and causes a significant reduction in MDA levels. However, similar results was not found in histopathologic findings.